Is it possible to perform mechanical measurements by making dozens of bioparticles “dance”? Quantitative Acoustophoresis (Greek motion by sound) employs acoustic waves to exert a wide range of forces on living cells and microscopic particles. Their movements in response to the acoustic field are tracked to calculate individual compressibilities, sizes, and densities. Doing that repeatedly ensures additional precision and enables tracking mechanical changes over time. View the article.

Studying cellular mechanics allows important insights into its cytoskeletal composition, developmental stage, and health. While many force spectroscopy assays exist that allow probing of mechanics of bioparticles, most of them require immobilization of and direct contact with the particle and can only measure a single particle at a time. Here, we introduce quantitative acoustophoresis (QAP) as a simple alternative that uses an acoustic standing wave field to directly determine cellular compressibility and density of many cells simultaneously in a contact-free manner. First, using polymeric spheres of different sizes and materials, we verify that our assay data follow the standard acoustic theory with great accuracy. We furthermore verify that our technique not only is able to measure compressibilities of living cells but can also sense an artificial cytoskeleton inside a biomimetic vesicle. We finally provide a thorough discussion about the expected accuracy our approach provides. To conclude, we show that compared to existing methods, our QAP assay provides a simple yet powerful alternative to study the mechanics of biological and biomimetic particles.